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KMID : 0364820090450020177
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Korean Journal of Microbiology
2009 Volume.45 No. 2 p.177 ~ p.184
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Probiotic Property of Lactobacillus pentosus Miny-148 Isolated from Human Feces
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Jung Min-Young
Park Yong-Ha
Kim Hyun-Soo
Poo Ha-Ryoung
Chang Young-Hyo
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Abstract
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Three hundred lactic acid bacteria isolated from human feces were studied their probiotic characters to develop potential probiotics. The properties were tested on the basis of guideline for probiotic selection protocol such as tolerance for acid or bile salt, thermal stability, antimicrobial, anticancer cell, and antiviral activity. Strain Miny-148 was selected as a potential probiotic bacterium which showed resistance to low pH, bile salts and thermal stability. On the basis of fatty acid profiles and 16S rDNA sequences analysis, the strain was identified as Lactobacillus pentosus (similarity 99.9%). The strain, L. pentosus Miny-148, showed broad antimicrobial spectrum against E. coli O157:H7, Shigella flexneri, Bacillus anthracis, Staphylococcus aureus, E. coli, Vibrio cholerae, V. vulnificus, Salmonella typhimurium, and Methicillin-resistant S. aureus (MRSA). Cell-free culture supernatant of the strain also inhibited against the growth of HT-29 colon cancer cell and transmissible gastroenterits virus.
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KEYWORD
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antimicrobial, identification, Lactobacillus, pathogenic, probiotic
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